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№ на артикул:
173738291
10X TAE Buffer (Tris-Acetate-EDTA), 250mL
№ на артикул:
173738291
€ 51
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Информация за продукта
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- 10X TAE (Tris-Acetate-EDTA) Buffer is used for running electrophoresis of nucleic acids in the gels for providing electrical conductivity and maintaining the pH. The EDTA present in the buffer inhibits metal dependent nucleases by chelating the divalent cations (Ca2+, Mg2+), and thereby protects the DNA from nucleases during the electrophoresis. The buffer may be used for both genomic and large supercoiled DNA, and also be as a gel preparation buffer.
- 10X Tris-EDTA Buffer (TE Buffer) is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation. May also be used as a washing buffer.
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.21 |
| Included Components | Bottle |
| Item Weight | 0.75 Pounds (340 grams) |
| Brand | eCon-Lab-Supply |
What's in the box
Bottle
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Въпроси на клиенти и отговори
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въпрос:
What is 10X TAE Buffer used for?
отговор: 10X TAE Buffer is primarily used for preparing agarose gels in nucleic acid analysis and electrophoresis procedures. It acts as a buffering agent that maintains a stable pH during electrophoresis, ensuring optimal separation of DNA and RNA molecules. It is also utilized in various molecular biology techniques, including cloning and gel extraction. By providing a conducive environment for nucleic acids, it enhances the resolution and reliability of laboratory experiments. -
въпрос:
How do I prepare 1X TAE Buffer from 10X TAE Buffer?
отговор: To prepare 1X TAE Buffer from 10X TAE, simply dilute the 10X solution with distilled water. Use a ratio of 1 part 10X buffer to 9 parts water to achieve the desired 1X concentration. For instance, if you need 100 mL of 1X TAE, mix 10 mL of 10X buffer with 90 mL of water. This dilution is crucial for maintaining the correct ionic strength during electrophoresis, which directly affects the migration speed of nucleic acids. -
въпрос:
What are the key components of 10X TAE Buffer?
отговор: The key components of 10X TAE Buffer include Tris base (tris(hydroxymethyl)aminomethane), acetic acid, and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent, regulating pH levels, while acetic acid facilitates the buffering capacity. EDTA chelates divalent metal ions, which is crucial for preventing the degradation of nucleic acids. These components work synergistically to foster optimal conditions for gel electrophoresis and molecular analysis. -
въпрос:
Can I use TAE Buffer for DNA or RNA electrophoresis?
отговор: Yes, 10X TAE Buffer is suitable for both DNA and RNA electrophoresis. Its buffering capacity allows for efficient migration and resolution of nucleic acids under electric fields. DNA and RNA can be analyzed effectively due to the stable pH environment provided by TAE. It is particularly advantageous for applications like restriction enzyme digestion and PCR fragment analysis, delivering high-quality results in various genomic studies. -
въпрос:
Is 10X TAE Buffer suitable for agarose gel electrophoresis?
отговор: Absolutely! 10X TAE Buffer is one of the most popular choices for agarose gel electrophoresis. Its composition helps maintain pH stability, which is vital for the accurate migration of nucleic acids during the gel electrophoresis process. Using TAE for agarose gels can enhance the resolution of bands, making it ideal for applications such as DNA separation, visualization, and downstream applications including cloning and sequencing. -
въпрос:
How should 10X TAE Buffer be stored?
отговор: 10X TAE Buffer should be stored at room temperature, away from direct sunlight and extreme temperature changes. Proper storage helps maintain its effectiveness and prolongs its shelf life. For maximum longevity, consider keeping it in a cool, dry place with minimal exposure to moisture. Before use, check for any precipitation, which may indicate degradation. Properly stored TAE Buffer remains effective for an extended period, ensuring reliable results in your experiments. -
въпрос:
What is the pH level of 10X TAE Buffer?
отговор: The standard pH level of 10X TAE Buffer is typically around 8.0. This alkaline pH provides a favorable environment for the stability of nucleic acids. It is crucial in maintaining the structural integrity of DNA and RNA during electrophoretic separation. A consistent pH is essential for effective electrophoresis, as it influences nucleic acid migration rates. Regular monitoring and calibration may be necessary in experimental setups to ensure optimal conditions. -
въпрос:
Can I use 10X TAE Buffer for blotting purposes?
отговор: Yes, 10X TAE Buffer can be used in certain blotting techniques, particularly Southern and Northern blotting, following electrophoresis. After the gel electrophoresis process, TAE is effective for transferring nucleic acids from agarose gels to membrane substrates. Its ionic composition aids in the efficient transfer of DNA or RNA, ensuring the integrity of the molecules during subsequent analysis, making it a versatile choice for molecular biology applications. -
въпрос:
Does 10X TAE Buffer contain any additives?
отговор: 10X TAE Buffer does not typically contain any additional additives such as tracking dyes or formamide. This purity is beneficial when you need a clean buffer for separation and analysis. However, it is common to add tracking dyes like bromophenol blue or xylene cyanol during gel preparation to monitor the progress of electrophoresis visually. These additives help ensure that runs are conducted effectively and can assist in determining the proper stopping point during the procedure. -
въпрос:
Where can I buy 10X TAE Buffer (Tris-Acetate-EDTA), 250mL in Bulgaria?
отговор: You can buy 10X TAE Buffer (Tris-Acetate-EDTA), 250mL on Ubuy. Ubuy is a popular e-commerce platform that offers a broad range of laboratory supplies, including buffers and reagents suitable for molecular biology. With its user-friendly interface and various payment options, Ubuy makes it convenient for you to acquire essential laboratory products efficiently.
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€ 51
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Характеристики и предимства
- Comprehensive range of biological buffers for various applications.
- Ideal for research, diagnostics, and drug development.
- Manufactured in an ISO certified facility in the USA.
- Rigorous quality control ensures high standards and reliability.
- 10X TAE and TE Buffer are critical for electrophoresis and molecular biology.
- Protects DNA and RNA from degradation and enhances research outcomes.