Tris-HCl Buffer, pH 9.4 [1M] - 100mL
€ 61
Price Details
Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )
*All items will import from САЩ
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Produced under the highest industry standards to ensure superior results.
Fast
Shipping
Free
Return*
Secure Packaging
100% Original Products
PCI DSS Compliance
ISO 27001 Certified
What Stands Out
Информация за продукта
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.53 |
| Included Components | Bottle |
| Item Weight | 4 ounces (113.4 grams) |
| Brand | eCon-Lab-Supply |
Who Should Buy?
-
Biochemistry Labs
Essential for maintaining stable pH conditions during biochemical assays and experiments in laboratory settings.
-
Molecular Biology
Ideal for DNA/RNA purification processes where a consistent pH is critical for enzymatic reactions.
-
Educational Institutions
Useful for teaching purposes in chemistry and biology classes, demonstrating buffer solutions and pH effects.
-
Acidic Reactions
Not suitable for experiments requiring acidic environments, as Tris-HCl is a basic buffer at pH 9.4.
-
Field Studies
Not practical for outdoor testing or field studies where controlled environmental conditions are absent.
-
Non-Aqueous Systems
Inappropriate for reactions or processes requiring non-water based solvents or media for optimal performance.
ОПИСАНИЕ НА ПРОДУКТА
Tris-HCl Buffer, pH 9.4 [1M] - 100mL
Въпроси на клиенти и отговори
-
въпрос:
What is Tris-HCl Buffer and why is it used?
отговор: Tris-HCl Buffer is a widely used buffering agent in biological and chemical applications, primarily due to its ability to maintain a stable pH environment. It is significant in molecular biology applications such as DNA and RNA extraction, electrophoresis, and enzyme assays. By stabilizing the pH, it prevents fluctuations that could compromise experimental results, ensuring consistency and reliability across experiments. -
въпрос:
What is the pH level of this Tris-HCl Buffer and its importance?
отговор: The Tris-HCl Buffer in question has a pH level of 9.4, which is essential for experiments requiring a slightly basic environment. This pH is particularly beneficial for reactions that involve nucleic acids and certain enzymes that operate optimally at higher pH levels. Maintaining this specific pH helps to improve reaction efficiency and accuracy in various laboratory procedures. -
въпрос:
Can I use Tris-HCl Buffer in protein purification?
отговор: Yes, Tris-HCl Buffer is commonly employed in protein purification processes. Its capacity to maintain a stable pH is critical during the various stages of protein extraction and purification, minimizing degradation and ensuring protein functionality. The buffer helps maintain solubility and activity of proteins throughout the purification process, making it a staple in biotechnology labs. -
въпрос:
How do I prepare Tris-HCl Buffer solutions?
отговор: To prepare Tris-HCl Buffer solutions, you can mix Tris base with hydrochloric acid (HCl) at the desired concentration. For a 1M Tris-HCl Buffer at pH 9.4, typically you need to dissolve 121.14 g of Tris base in distilled water, then adjust the pH with concentrated HCl. This precise adjustment ensures that the buffer maintains its target pH for experimental use. -
въпрос:
Is Tris-HCl Buffer suitable for cell culture?
отговор: Yes, Tris-HCl Buffer can be used in cell culture applications, but it is often combined with other medium components to enhance nutrient availability and maintain pH balance. Its use is prevalent when the exact pH is crucial for cellular activities, such as enzyme functions or metabolic processes, facilitating optimal cell growth. -
въпрос:
What are the advantages of using Tris-HCl Buffer over other buffers?
отговор: Tris-HCl Buffer offers several advantages, such as a wide buffering range and minimal salt concentration, which is less likely to interfere with biological reactions. Additionally, its relative stability at various temperatures makes it suitable for many laboratory conditions. These properties make Tris-HCl Buffer a preferred choice over other buffering agents in many molecular biology applications. -
въпрос:
What applications are best suited for this specific concentration of Tris-HCl Buffer?
отговор: The 1M concentration of Tris-HCl Buffer at pH 9.4 is particularly useful in applications involving DNA and RNA manipulation, such as PCR and gel electrophoresis. This concentration helps maintain nucleic acid stability while allowing enzymatic reactions to proceed efficiently, making it essential in both academic and research labs focused on nucleic acid studies. -
въпрос:
Can Tris-HCl Buffer be stored, and how long does it last?
отговор: Tris-HCl Buffer can be stored at room temperature and should have a relatively long shelf life if kept away from direct sunlight and high temperatures. However, it’s optimal to check for any visible signs of degradation or contamination prior to use. For best results, storing the buffer in tightly sealed containers helps prolong its usability for critical experiments. -
въпрос:
Is this buffer compatible with other reagents commonly used in laboratories?
отговор: Tris-HCl Buffer is compatible with a variety of common laboratory reagents, including salts, nucleotides, and proteins. However, when using it with sensitive enzymes or compounds, you should verify compatibility, as pH can impact their stability. It’s often mixed with other buffers or reagents to create tailored environments for specific reactions, making it versatile in various protocols. -
въпрос:
Where can I buy Tris-HCl Buffer, pH 9.4 [1M] - 100mL?
отговор: You can conveniently purchase Tris-HCl Buffer, pH 9.4 [1M] - 100mL from Ubuy, a reliable online marketplace that offers a variety of laboratory supplies. Ubuy provides an efficient shopping experience for scientists and researchers looking for quality reagents and chemicals, ensuring you have access to the necessary tools for your work.
eConLabSupply Buffers Editorial Review
Customer Reviews & Ratings
-
5 звезда
100%
-
4 звезда
0%
-
3 звезда
0%
-
2 звезда
0%
-
1 звезда
0%
Дайте отзив за този продукт
Споделете вашите мисли с други клиенти
Product Price History
Важна информация
- Ограничения: За продукти, доставени в международен план, имайте предвид, че е възможно не всяка гаранция на производителя да е валидна; опциите за обслужване на производителя може да не са налични; ръководствата на продукта, инструкциите и предупрежденията за безопасност може да не са на езиците на държавата на местоназначение; продуктите (и придружаващите ги материали) може да не са проектирани в съответствие със стандартите, спецификациите и изискванията за етикетиране на страната по местоназначение; също така продуктите може да не отговарят на напрежението в държавата по местоназначение и на други електрически стандарти (изискващи използване на адаптер или преобразувател, ако е необходимо). Получателят е длъжен да гарантира, че продуктът може да бъде внесен законно в страната по местоназначение. Когато поръчвате от Ubuy или неговите партньори, получателят е вносител и трябва да спазва всички закони и разпоредби на страната по местоназначение.
- Не всички продукти, изброени в Ubuy, се продават, тъй като Ubuy е глобална търсачка. Продуктите са предмет на разпоредбите за износ/търговия.
€ 61
Поръчайте сега и я вземете Събота, Юли 04
This item is not restrict in my country.(Please click on above link if this item is not restrict in your country, So our team will review and allow.)
QTY:
Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
Характеристики и предимства
- Manufactured in an ISO 9001 and 13485 certified facility in the USA.
- Stringent Good Manufacturing Practices followed for quality assurance.
- Aseptic filtration ensures product integrity and purity.
- Ideal for molecular biology applications, especially nucleic acid purification.
- Complete Certificate of Analysis provided for every production lot.
- Can be easily diluted to achieve desired concentrations.