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№ на артикул:
161477029
TE Buffer, Tris-EDTA Buffer (1) pH 8.0, 500mL, Single Bottle
№ на артикул:
161477029
€ 16
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This Tris-EDTA Buffer (1×) is precisely adjusted to pH 8.0, providing consistent and reliable conditions for a variety of laboratory procedures.
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What Stands Out
Consistent pH Level
This TE Buffer maintains a stable pH of 8.0, ensuring optimal performance in nucleic acid storage and analysis, minimizing variability and improving reproducibility in experiments.
Convenient Packaging
Supplied in a ready-to-use 500mL single bottle, this TE Buffer eliminates the need for preparation, saving valuable time and ensuring precision in laboratory work.
Versatile Applications
Ideal for molecular biology, this TE Buffer is perfect for DNA/RNA stabilization, enabling researchers to conduct a wide range of experiments with confidence in their results.
Информация за продукта
| Item Weight | 1.2 lbs (540 grams) |
Who Should Buy?
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Molecular Biologists
Ideal for DNA and RNA extraction and storage, providing stability and protection against degradation during experiments.
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Genomic Research
Supports buffer usage in PCR, sequencing, and cloning applications critical for genomic studies and experiments.
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Laboratory Educators
Suitable for educational settings, enabling students to learn buffer applications in molecular biology and biochemistry classes.
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Protein Work
Not suitable for protein purification and analysis due to the potential interference of Tris with protein assays.
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Field Use
Not practical for portable fieldwork due to its size and sensitivity to environmental conditions outside the lab.
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Non-biological Research
May not meet the needs of researchers in disciplines outside molecular biology, such as materials science or physics.
ОПИСАНИЕ НА ПРОДУКТА
TE Buffer, Tris-EDTA Buffer (1) pH 8.0, 500mL, Single Bottle
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Въпроси на клиенти и отговори
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въпрос:
What is TE Buffer and what is its primary function?
отговор: TE Buffer, which stands for Tris-EDTA Buffer, is a common laboratory solution used primarily to maintain the pH of nucleic acid samples and stabilize them against degradation. Its pH of 8.0 makes it ideal for a variety of molecular biology applications, including the storage of DNA and RNA. By utilizing both Tris as a buffering agent and EDTA as a chelator that sequesters divalent metal ions, TE Buffer helps prevent nucleases from degrading nucleic acids, making it essential for sample preservation and manipulation. -
въпрос:
How is TE Buffer typically used in molecular biology?
отговор: TE Buffer is frequently used in molecular biology for storing and diluting nucleic acids, such as DNA and RNA. By maintaining a stable pH, it ensures the integrity of the samples during processes like PCR, sequencing, and cloning. Additionally, it's often used in preparation for electrophoresis and other analytical techniques, providing optimal conditions for nucleic acid interactions. For instance, researchers might use TE Buffer to resuspend DNA after extraction to maintain its stability during downstream applications. -
въпрос:
Is the TE Buffer provided sterile, and how should it be stored?
отговор: Yes, the TE Buffer is typically provided in a sterile single bottle to ensure that it is free of contaminants. For optimal preservation, it is recommended to store the buffer at room temperature away from direct sunlight, and if long-term storage is required, refrigeration may be considered. This will help maintain the buffer's effectiveness, as temperature fluctuations could potentially affect its stability over time. Ensuring sterility is crucial when working with sensitive biological samples. -
въпрос:
Can TE Buffer be used for both DNA and RNA samples?
отговор: Absolutely! TE Buffer is suitable for both DNA and RNA samples, making it a versatile solution in molecular biology. Its neutral pH and protective properties make it ideal for preserving the integrity of both types of nucleic acids during storage and handling. For example, when preparing RNA for applications like reverse transcription qPCR, TE Buffer ensures the stability of the sample, which is vital to obtaining accurate results. -
въпрос:
What are the components of TE Buffer and their significance?
отговор: TE Buffer consists mainly of Tris (tris(hydroxymethyl)aminomethane) and EDTA (ethylenediaminetetraacetic acid). Tris acts as a buffering agent, helping to maintain a stable pH during experiments, while EDTA chelates divalent metal ions, which can catalyze the degradation of nucleic acids. The synergy between these components not only stabilizes nucleic acids but also keeps them safe from enzymatic activities that could compromise research findings, making it indispensable in genetic analysis. -
въпрос:
Is it possible to make TE Buffer at home, and if yes, how?
отговор: Yes, you can prepare TE Buffer at home by mixing the appropriate amounts of Tris and EDTA in distilled water and adjusting the pH to 8.0 with hydrochloric acid (HCl). Typically, a common recipe is to use 10 mM Tris and 1 mM EDTA. It's essential to ensure all glassware and equipment used are sterile to prevent contamination. While homemade buffers can be effective, ready-made TE Buffer ensures consistent quality and saves time, especially in high-throughput labs. -
въпрос:
What are the benefits of using TE Buffer over other buffers?
отговор: TE Buffer offers several advantages over other buffering systems, primarily its ability to stabilize nucleic acids against degradation. It is pH-stable, ensuring that the nucleic acids remain intact for extended periods, which is critical for applications requiring high fidelity. In contrast to other buffers that may not be optimal for nucleic acid work, TE Buffer's unique formulation is recognized for its role in enhancing the stability and reliability of molecular biology experiments, making it a necessary choice in many lab settings. -
въпрос:
Can TE Buffer be used in conjunction with other reagents in experiments?
отговор: Yes, TE Buffer can be effectively used with a variety of reagents in molecular biology experiments without interfering with their functionality. It is commonly used during processes such as DNA amplification, fragment analysis, and cloning, where it can be supplemented with enzymes, primers, and other reagents. For example, when setting up a PCR reaction, using TE Buffer ensures that the DNA template remains stable and that enzymatic activities are maintained during the reaction cycles. -
въпрос:
What should I do if I notice a color change in my TE Buffer?
отговор: If you notice a color change in your TE Buffer, it may indicate contamination or degradation. Normally, TE Buffer is a clear solution; any discoloration could suggest microbial growth or chemical reactions. It's advisable to discard any buffer that looks different than expected to ensure experimental integrity. Always use sterile techniques when handling the buffer and ensure it is stored properly to minimize the risk of contamination. Using newly prepared or commercially available TE Buffer can help avoid such issues. -
въпрос:
Where can I buy TE Buffer, Tris-EDTA Buffer 1 pH 8.0, 500mL, Single Bottle?
отговор: You can purchase TE Buffer, Tris-EDTA Buffer 1 pH 8.0, 500mL, Single Bottle at Ubuy in Bulgaria. Ubuy provides a reliable platform where you can find a variety of laboratory supplies, ensuring you receive quality products delivered to your location. Their inventory is designed to cater to both individual researchers and larger institutions needing essential chemical solutions for their experiments.
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Професионалисти
- High-quality pH stability
- Ready to use
- Excellent for molecular biology
- Compatible with various applications
- No need for additional preparation
минуси
- The bottle design could be improved.
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Важна информация
- Ограничения: За продукти, доставени в международен план, имайте предвид, че е възможно не всяка гаранция на производителя да е валидна; опциите за обслужване на производителя може да не са налични; ръководствата на продукта, инструкциите и предупрежденията за безопасност може да не са на езиците на държавата на местоназначение; продуктите (и придружаващите ги материали) може да не са проектирани в съответствие със стандартите, спецификациите и изискванията за етикетиране на страната по местоназначение; също така продуктите може да не отговарят на напрежението в държавата по местоназначение и на други електрически стандарти (изискващи използване на адаптер или преобразувател, ако е необходимо). Получателят е длъжен да гарантира, че продуктът може да бъде внесен законно в страната по местоназначение. Когато поръчвате от Ubuy или неговите партньори, получателят е вносител и трябва да спазва всички закони и разпоредби на страната по местоназначение.
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€ 16
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Характеристики и предимства
- Stable pH 8.0 for consistent experimental results.
- Ready-to-use with no dilution needed, saving time.
- Versatile for various lab techniques such as DNA, RNA, and protein analysis.
- High-quality formula ensures long-lasting stability and performance.
- Designed for easy storage, minimizing contamination risk.
- Convenient packaging enhances accessibility in the lab.
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